January 1, 2018 (Vol. 38, No. 1)
MilliporeSigma
The Clarisolve® mPAA polymer is a stimulus-responsive flocculation polymer designed specifically to enable a simpler clarification of high-density cell culture monoclonal antibody (mAb) processes and provides an alternative to centrifugation for these challenging bioreactor harvests when used in combination with Clarisolve depth filters. High cell-density feed streams typically require increased depth-filtration area. Although flocculation reagents such as acids or polymers can help reduce the filtration area needed to clarify these streams, some chemicals can negatively affect the stability of mAbs or leave high residual levels post-flocculation. The Clarisolve mPAA polymer achieves the reduction in filtration area while minimizing risk by providing low residual polymer levels with no negative impact on downstream unit operations. Compared with other flocculation polymers, the flocculation with the Clarisolve mPAA polymer is less dependent on the dosage of the polymer, which increases robustness and results in a wide dosing window.
