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November 15, 2013 (Vol. 33 , No. 20)

Stain-Free Gel Visualization Application

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The lighting and filter conditions in the G:BOX XR5 image analysis system can be adjusted to allow the visualization of proteins on stain-free gels. This G:BOX XR5 application was evaluated in tests conducted with a Criterion TGX stain-free gel that had been exposed to five minutes of mid-wave UV (302 nm) then imaged using a UV filter and an exposure time of two seconds. The protein imaging made possible by this application was reportedly comparable to that achieved using gels that had been stained with ProtoBlue Safe.

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