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January 1, 2017 (Vol. 37 , No. 1)

RP and IEX Columns for Synthetic Oligonucleotides Analysis

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Two new particle chemistries have been launched: the Clarity Oligo-XT, for high-efficiency reversed-phase LC analysis and purification, and Clarity Oligo-SAX, a high-resolution strong anion exchanger (SAX) for characterization. The new Clarity Oligo-XT C18 columns feature novel and robust core-shell media, for high-efficiency reversed-phase characterization of synthetic DNA and RNA, pH stability from 1 to 12 and increased sensitivity that improves quantitation by mass spectrometry. Clarity Oligo-XT columns are available in directly scalable 1.7 µm, 2.6 µm, and 5 µm particle sizes that enable easy method transfer between analytical HPLC/UHPLC instrumentation and preparative purifications systems. The Clarity Oligo-SAX columns feature an entirely new, rugged nonporous particle that retains synthetic oligonucleotides through strong ion exchange mechanisms, adding a robust strong anion exchanger choice with improved column lifetimes to the Clarity family. These nonporous particles are engineered for performance at high pH (2.5 to 12.5) and temperatures up to 85°C and are provided in 5 µm particle size for analytical characterization.

  • Phenomenex
  • 411 Madrid Avenue
  • Torrance CA, 90501

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