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December 1, 2013 (Vol. 33 , No. 21)

Bioluminescent H<sub>2</sub>O<sub>2</sub> Assay

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The ROS-Glo™ H2O2 Assay is a homogenous, nonhorseradish peroxidase-dependent, plate-based bioluminescent assay that measures the level of H2O2 directly in cell culture or in defined enzyme reactions. A derivatized luciferin substrate is incubated with the sample and reacts directly with H2O2 to generate a luciferin precursor. Addition of a detection solution converts the precursor to luciferin and provides a recombinant luciferase to produce a light signal that is proportional to the level of H2O2. The assay, which is performed following a two-reagent-addition protocol and does not require sample manipulation, can be completed in less than two hours after reagent addition.

  • Promega
  • 2800 Woods Hollow Road
  • Madison WI, 53711

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