Integrating Pathology and Next-Gen Sequencing for a Robust and Practical Workflow
- Broadcast Date:
Wednesday, December 4, 2013
11:00 am ET, 8:00 am PT
REGISTRATION IS FREE
Next-generation sequencing (NGS) technologies have provided invaluable research tools for determining the genetic basis of disease. Enhanced genetic analyses present pathologists and clinical genomics facilities with unique sample-prep and workflow challenges. The nature and purity of tumor samples used as a source of DNA for NGS may profoundly influence interpretation of the sequencing output.
Clinical samples for whole-genome sequencing may be of suboptimal size, contain necrotic tumor, have high stromal or nontumoral content, or yield fragmented DNA. These limitations have prompted the development of novel experimental approaches and computational methods in order to obtain accurate second-generation sequencing data. Refinement of bioinformatic techniques to assess the significance of a somatic mutation in the context of the factors such as the background mutation rate, tumor ploidy, and stromal contamination for primary cancers also remains a major challenge of NGS.
Approaches to addressing these challenges include methods for sample preparation that benefit from pathologists’ understanding of tissue morphology and for data fusion, but that have not yet been adequately integrated into a practical, standardize, and clinically robust workflow.
Participants in this webinar will describe methods for optimal tissue collection and preservation to ensure robust samples for sequencing.
What You Will Learn
- How leading pathologists are employing NGS into clinical workflows
- How NGS data is used in conjunction with anatomical pathology to maximize utility of limited sample
- How PerkinElmer’s solutions for automated sample preparation provide the throughput and reproducibility necessary to translate NGS tests into clinical pathology laboratories
Who Should Attend
- Pathologists using NGS for tissue and tumor analyses
- Core NGS facility managers
- NGS sequencing scientists
- Clinical genomics scientists
- Pathology laboratory managers
A live Q&A session will follow the presentations,
offering you a chance to pose questions to our expert panelists.