Inline Automation and Miniaturized Reaction Volumes for Real Time Isothermal DNA Amplification
- Broadcast Date:
Wednesday, July 17, 2013
1:00 PM ET, 10:00 am PT
REGISTRATION IS FREE
Real-time polymerase chain reaction (qPCR) processing can be a labor-intensive process involving multiple instruments and numerous human (or robotic) contact points with each microplate. Douglas Scientific has tamed this cumbersome process for high-throughput laboratories through the creation of a fully automated, inline data-generating system that utilizes very low volume reactions. In this webinar, Douglas Scientific will introduce the Nexar® Optimized for Real Time Isothermal DNA Amplification. When this system is combined with the isothermal DNA amplification chemistry developed by EnviroLogix, labs can go from sample to answer in 20 minutes or less.
The Douglas Scientific Array Tape™ Platform has been optimized for isothermal chemistries, providing a high-throughput alternative to traditional PCR analysis. Array Tape is a continuous polymer strip, serially embossed with reaction wells in customized volumes and formats. Array Tape is thin (0.3 mm) and flexible, which allows 200 microplate equivalents (76,800 reaction wells) to be spooled onto a single, compact reel (90 mm wide by 560 mm diameter). It’s a highly automated, flexible microplate replacement for high throughput applications that allows for a streamlined laboratory process.
DNAble® chemistry enables several billion-fold amplification in 10 minutes or less at a constant temperature with high specificity, and sensitivity resulting in reproducible, high quality data. This isothermal nucleic acid amplification technology eliminates the need for a thermal cycler. When combined with the low reaction volumes and automation of the Array Tape Platform, it offers a lower cost, faster, and flexible method of genetic analysis.
In this webinar, scientists from Douglas Scientific, manufacturer of the Array Tape platform, and EnviroLogix, provider of DNAble chemistries, will discuss how the merging of these technologies addresses many challenges of real time nucleic acid processing, gene expression, RT-PCR, and qPCR.
What You Will Learn
- How the Nexar Optimized for Real Time Isothermal DNA Amplification provides an inline, automated qPCR alternative with increased throughput, decreased reagent costs, and true walk away time
- How the DNAble isothermal DNA amplification chemistry dramatically reduces the time required for amplification and real time data generation
- How the combination of increased amplification speed, inline automation, and miniaturized reaction volumes increase throughput and decrease cost per data point for real time nucleic acid processing
Who Should Attend
- Environmental scientists
- Molecular biologists
- Agricultural molecular biologists
- Microbiologists Genomics scientists
- Genomics laboratory managers
A live Q&A session will follow the presentations,
offering you a chance to pose questions to our expert panelists.