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GEN Presents An Educational And Informative Webinar

How Ion Mobility Coupled with High-Resolution Mass Spec Enables Analysis of Complex Samples

  • Broadcast Date: Tuesday, March 25, 2014
  • Time: 11 am ET, 8 am PT, 4 pm CET; 2nd Presentation: 1 pm ET, 10 am PT



Despite significant advances in mass spectrometry (MS) technology, obtaining high-quality data with structural fidelity and comprehensive coverage of compounds with high dynamic ranges in complex matrices remains challenging. This limitation results in the inability to effectively account for subtle variations reflecting biodiversity and structural differences.

Recently, ion mobility (IM) separation has been combined with MS to afford greatly improved peak capacity and selectivity by combining the separation powers of LC, IM, and MS techniques. Laboratories involved in cutting-edge research can speed up research programs and have greater confidence in compound identification with the additional dimension of mobility separation as well as the structural information.

Ion mobility-mass spectrometry provides gas-phase mobility separations on the basis of charge, size, and shape of the molecule and is well suited for integration with high-resolution, accurate mass measurements. The gas-phase ion separation technique is carried out in a drift tube filled with an inert buffer gas and energized with a low static electric field. Larger ions traverse the drift tube at a slower velocity than smaller ions resulting in gas-phase ion separation. Current instrumentation maintains excellent ion transmission efficiency for both IM and QTOF modes of operation while maintaining high resolution in both dimensions.

Further, users say, timescales of this multidimensional separation are well suited for combination with fast condensed-phase separations, such as UHPLC, for enhanced separation selectivity as sample complexity becomes more challenging.

In this webinar experts will describe recent advances in IM-MS measurement strategies in the analyses of complex biological samples in systems biology, proteomics, and protein characterization. New advances in bioinformatics and biostatistics to quickly mine the data gathered to provide targeted and actionable information will also be described. Other applications of IM-MS and instrumentation in high-throughput analysis of biomolecule mixtures will be presented.

Who Should Attend

  • Analytical chemists
  • Protein structure researchers
  • Chemical biologists
  • Scientists in proteomics, metabolomics, and other omic areas
  • Mass spectrometrists

You Will Learn

  • How IM-MS provides rapid gas-phase electrophoretic separations on the basis of molecular structure
  • How coupling an IM separation with MS affords greatly improved measurement throughput, sensitivity, robustness, and quantitative capabilities for rapid analysis of complex samples
  • How benchmark studies of complex sample analysis including lipid-specific extractions from rat spinal fluid were performed and analyzed using LC-QTOF and LC-IM-QTOF techniques that resulted in detection of additional features, which indicated the increase in peak capacity provided by IM
  • How data resulting in drift time aligned fragment ion spectra with lower chemical noise are easier to interpret than conventional MS/MS spectra

A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.



  • John A. McLean, Ph.D.
  • Stevenson Associate Professor of Chemistry
  • Vanderbilt University
  • Erin Shammel Baker, Ph.D.
  • Senior Research Scientist
  • Pacific Northwest National Laboratory
  • George Stafford, Ph.D.
  • Director of LC/MS R&D
  • Agilent Technologies


  • Tamlyn Oliver
  • Managing Editor
  • Genetic Engineering & Biotechnology News

Produced with support from