Efficiency Gains with Sequence Variant Analysis by Mass Spectrometry
- Broadcast Date:
Wednesday, January 22, 2014
11:00 am ET, 8:00 am PT
REGISTRATION IS FREE
Mass spectrometry has become an important tool to characterize protein therapeutics. In order to develop a protein therapeutic it is critical to fully understand both the protein sample itself and the process leading to its generation. The clone selection process involves analyzing the produced protein product to confirm the amino acid sequence is correct and determine which leads to pursue.
Sequence Variant Analysis (SVA) is an important component of biological product characterization and clone selection. Amino acid mis-incorporation in protein products occurs due to either DNA mutations or RNA transcriptional or translational errors. Although the impact of amino acid sequence variants on the properties of a protein therapeutic can be varied, it is important to identify and catalog them so corrective actions may be taken if necessary.
In the past, the task of seeking out sequence variants has been extremely time-consuming and challenging from an analytical point of view because of the enormous number of theoretical possibilities, especially for proteins such as monoclonal antibodies. Other challenges include the need to both observe and identify amino acid sequence variants that may be at very low abundance in the biological product or in highly complex samples. Sequence variants are often identified through peptide mapping experiments where the protein product is compared against either a standard protein lot or one from another expression clone.
The development of modern mass spectrometry instrumentation, such as the TripleTOF® 5600+, that provides both high resolution and accurate mass information over a wide dynamic range is able to generate data to assist in the characterization of biologics and post-translational modifications. Generating high-quality data is only part of the story as the need for processing tools to assist in turning the data into useful information is just as important. The database search program ProteinPilot™ can provide necessary efficiencies in data analysis when looking for amino acid sequence variants and post-translational modifications.
In this webinar we will explore some examples where ProteinPilot™ was used to identify protein sequence variants present in cell line protein products even in the absence of a protein standard. ProteinPilot™ can also be used to identify amino acid sequence differences in antibodies that are a result of somatic mutations during antibody affinity maturation.
What You Will Learn
- How modern mass spectrometry and informatics can provide efficiency gains in Sequence Variant Analysis
- How the continual use of accurate mass data with informatics can inform the clone selection process
- How LC-MS can be incorporated into clone selection processes
Who Should Attend
- Protein and peptide biochemists
- Recombinant protein and monoclonal antibody therapeutics developers
- Therapeutic protein manufacturing process development scientists
- Cell culture development departments
- Process development scientists
- Biosimilar developers
- Biotherapeutics developers who use, or who rely on, mass spectrometry
A live Q&A session will follow the presentations,
offering you a chance to pose questions to our expert panelists.