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GEN Presents An Educational And Informative Webinar

Automated Mass Spectrometry Workflows for High-Throughput Bioanalysis of Proteins with SISCAPA

  • Broadcast Date: Wednesday, May 7, 2014
  • Time: 11:00 am ET, 8:00 am PT



Coupling immunoaffinity enrichment using antipeptide antibodies (SISCAPA) to multiple reactions monitoring mass spectrometry (MRM-MS) enables multiplexed quantification of large numbers of proteins in biological samples with precision, sensitivity and high throughput.

Originally developed to increase the throughput and detection limits of biomarker candidates, the method is valuable for bioanalysis of biologics since it is immune to the anti-drug antibody interferences of ligand-binding assays.

The low signal to noise facilitates using a rapid bind/elute process such as Agilent RapidFire MS in place of the time-consuming reversed-phase separation now used as a prelude to online MS peptide assays. 

Webinar participants will explore a highly automated SISCAPA-MS workflow and simple user interface developed on the Agilent Bravo Liquid Handling Platform and discuss specific applications of this technology.

Who Should Attend

  • Bioanalytical chemists responsible for PK studies of biologics and small molecules.
  • Researchers quantitating small molecules or proteins from dried blood spots.
  • Investigators carrying out multiplexed protein quantitation.
  • Developers of therapeutic antibodies, proteins, or small molecules.
  • Scientists conducting ELISA or ligand-binding assays.
  • Biomarker researchers focused on candidate validation and verification.

You Will Learn

  • How a SISCAPA-MS workflow developed on the Agilent Bravo Liquid Handling Platform can be used in a multiplexed manner for simultaneous quantitation of therapeutic proteins and circulating disease biomarkers.
  • How the same workflow can be applied across species for preclinical pharmacokinetic analysis through to therapeutic drug monitoring.
  • How to improve peptide multiple reaction monitoring (MRM) sensitivity by 3 to 4 orders of magnitude over nonenriched samples.
  • How to employ true internal standards (stable isotope labeled synthetic peptides) within each assay for reliable quantitation.
  • How assays can be combined in mix-and-match panels without cross-reactions common in sandwich immunoassays.
  • How to deliver highly purified peptide analytes, free of matrix components, for decreased LC times and higher throughput.
  • How to allow faster, less expensive, and more straightforward assay development than sandwich immunoassay development.

A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.



  • N. Leigh Anderson, Ph.D.,
  • Founder
  • SISCAPA Assay Technologies
  • Rachel Bolger,
  • Product Manager
  • Agilent Technologies


  • John Sterling
  • Editor in Chief
  • Genetic Engineering & Biotechnology News

Produced with support from