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GEN Webinars: January 01, 0001 (Vol. , No. )

Decoding Transcriptional Regulation by Genome-Wide Reporter Assays and Large-Scale Transfection

<p> Despite our knowledge of the complete genome sequences of several dozen species and high-quality annotation of the protein coding genes, the identification of active regulatory elements remains challenging, especially for distal enhancers. Traditional methods to measure enhancer activity directly are limited by throughput, so conventional approaches depend largely on indirect methods that profile features correlated with regulatory elements including the chromatin landscape and transcription factor binding sites. In this GEN webinar, we will learn about two novel reporter assays, STARR-seq and STAP-seq, ectopic, plasmid-based, massively parallel reporter assays that can directly and quantitatively measure the activity of millions of candidate sequences for enhancers and core promoters. Moreover, we will learn how these reporter assays were enabled by MaxCyte’s scalable flow electroporation technology to attain high transfection efficiencies of plasmid reporter libraries—key to the reporter assays’ success.</p> <h3> Who Should Attend</h3> <ul> <li> Gene expression researchers</li> <li> Scientists interested in large-scale transfection</li> <li> Molecular biologists</li> <li> Transcriptomic scientists</li> <li> Genomics and epigenetic researchers</li> </ul> <h3> You Will Learn</h3> <ul> <li> How high-throughput transcriptional reporter assays coupled to large-scale transfection of cultured cells, enables the study of transcriptional regulation on a genome-wide scale</li> <li> About the STARR-seq and STAP-seq methods, two technologies developed to study genome-wide enhancer and core promoter activity</li> <li> About new results obtained by applying these methods to different questions in gene regulation in the Drosophila S2 cell model</li> </ul>