Two iTaq kits have been introduced for reverse transcription quantitative PCR (RT-qPCR) applications. They are designed for use under most reaction conditions on any qPCR instrument. The kits include the iTaq universal SYBR® Green one-step kit and the iTaq universal probes one-step kit. Both kits come as a 2x-concentrated reaction mix. The multiple passive reference dyes included in their formulation may be useful for researchers using qPCR instruments that require ROX fluorescent dyes. In addition, the combination of a reverse transcriptase and an antibody-mediated DNA polymerase in one reaction is meant to improve PCR performance.