Precision Blue™ real-time PCR dye is a concentrated, ready-to-use reagent that increases the visibility of real-time PCR reaction mixtures. The formulation facilitates precise pipetting and accurate reaction tracking when loading tubes or plates for real-time qPCR experiments. It could be particularly useful when loading white or 384-well plates. The blue dye improves the visibility of qPCR reaction mixtures, enabling loading of small volume reactions with greater speed, accuracy, and precision. Applications such as single nucleotide polymorphism genotyping and high-throughput gene expression analysis may benefit from use of this dye. Precision Blue dye does not interfere with SYBR® Green- or probe-based detection. The dye can be used with all real-time PCR systems. For convenience, it can be added directly tothe supermix or used as a component of a reaction master mix. The dye is concentrated 200x, and delivers 500 x 20 μL reactions.