GEN Exclusives

More »

New Products

More »
Jun 1, 2014 (Vol. 34, No. 11)

EMD Millipore

Click Image To Enlarge +

Protein Purification Protocol

The new recombinant protein purification protocol combines lysis and purification into a single step. The condensed workflow can be completed in about 45 minutes, reducing processing time by 75% compared to traditional techniques, while delivering high yield and purity. The method uses a specialized lysis buffer in combination with magnetic affinity capture beads, which together eliminate the need to clarify lysates by centrifugation.  As a result, the workflow can be automated using a high-throughput particle processor, further reducing sample variability. The one-step lysis and purification protocol has been validated specifically for PureProteome™ Nickel Magnetic Beads, optimized for the capture of histidine-tagged proteins, and BugBuster® Master Mix, a lysis reagent that allows for nonmechanical extraction of soluble protein from bacterial cells. This reagent combines detergent-based lysis with enzymatic agents, Benzonase® nuclease, and rLysozyme™ enzyme, in a ready-to-use formulation.

  • EMD Millipore
  • 290 Concord Road
  • Billerica, MA, 01821

Related To This Company





GEN Jobs powered by connects you directly to employers in pharma, biotech, and the life sciences. View 40 to 50 fresh job postings daily or search for employment opportunities including those in R&D, clinical research, QA/QC, biomanufacturing, and regulatory affairs.
More »

Be sure to take the GEN Poll

Cancer Drug Approvals and Efficacy

Do you think regulatory agencies should be required to provide stronger evidence of the clinical effectiveness of cancer drugs, including a greater emphasis on patient survival time?

More »