FluoScout is an online tool that can recommend a filter cube, or set of optical filters, to users of fluorescence microscopes. Once a filter cube is matched with a light source and fluorophores, it is possible to maximize the excitation and efficiency of fluorescent dyes or proteins. To gauge the range of visible light, the user can view the FluoScout display, which shows the spectrum as a colored bar. To obtain an overview, the user can fade in and out of single spectra. To see more details, the user can use a zoom feature. Transmission and emission can also be quantified.