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Nov 15, 2013 (Vol. 33, No. 20)

NanoTemper Technologies

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Microscale Thermophoresis Instrument

The Monolith NT.115Pico is a device for the immobilization-free measurement of high-affinity binding interactions. It assesses microscale thermophoresis (MST), which is the directed motion of molecules through a local temperature gradient. (Relative changes of movement along a temperature gradient may be used to determine binding affinities and energetics.) The instrument can detect a dynamic range of pM to mM dissociation constants, generating results in 10 minutes while consuming less than 4 µL of sample. It is designed to monitor the binding of single ions or small molecules to a target as well as the binding of ribosomes.

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