A new 48-bioreactor version of the microbioreactor system ambr™ is now available for more complex experimental designs. This new version of the ambr workstation automates the operation of 48 disposable microbioreactors and closely replicates the characteristics of benchtop bioreactors at microscale (10–15 mL working volume). In the new system, the 48 micro bioreactors are arranged in four culture stations containing sets of 12, each with a miniature internal impeller, as well as individual closed-loop control of dissolved O2 and pH and a supply of gases. Each culture station has independent stirring speed and temperature control, allowing scientists to test up to four different temperature and stir speeds simultaneously.