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August 1, 2017 (Vol. 37 , No. 14)

Membrane Chromatography Cassettes

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Sartorius Stedim Biotech

The new Sartobind® cassettes offer the same flowpath, bed heights (4 and 8 mm), and void volume ratios as Sartobind capsules, and are compatible with Q, S, STIC PA, and phenyl ligands. The new design goes beyond the previous 5-L size limitation for capsule formats, expanding the boundaries of membrane chromatography. Multiple cassettes, each with 0.8-L or 1.6-L membrane volume, can be set up in three different stainless-steel holders, resulting in maximum membrane volumes of 20, 50, or 100 L, respectively. Data demonstrate direct scalability from the 3 mL Sartobind nano capsule to 20.8 L (13 cassettes) in the Pilot Filter Holder. Pressure-flow performance and the shape of breakthrough curves are identical to the smaller capsule sizes, independent of the number of cassettes used.

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