The HumanKine G4 dendritic cell generation kit can yield DCs from peripheral blood mononuclear cells with no medium changes. This protocol is enabled by HumanKine GM-CSF and HumanKine IL-4, which are included in the kit and produced in a human cell expression system. Unlike nonhuman cell-expressed GM-CSF and IL-4, the HumanKine cytokines possess native human glycosylation and have good stability, half-life, and bioactivity.