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July 1, 2017 (Vol. 37 , No. 13)

Bioreactor with Automated Cell-Culture Analyzer

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Sartorius Stedim Biotech

The ambr® 15 automated microbioreactor system combined with a Nova Biomedical (Nova) BioProfile® FLEX2 automated cell culture enables rapid at-line sample collection and analysis of massive quantities of key cell-culture data. The combined system provides a unique tool to simultaneously run, sample, and analyze a massive number of cell-culture conditions during cell-line and media development by design of experiments (DoE). The integrated software enables automated, parallel processing of 24 or 48 single-use bioreactors (10–15 mL working volume). This allows collection of enormous quantities of cell-culture data, including total and viable cell density, viability, cell diameter, pH, PCO2, PO2, glucose, lactate, glutamine, glutamate, ammonium, Na+, K+, Ca++, and osmolality, which can be sampled and measured with a complete cycle time of just 6–7 minutes.

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