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Sep 15, 2013 (Vol. 33, No. 16)

AMS Biotechnology

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Aptamer Protein Precipitation Kits

Aptoprecipitation (AP)/Co-aptoprecipitation (Co-AP) kits are aptamer-based protein precipitation kits designed to bind with high specificity and affinity to target molecules. In these kits, aptamer is covalently conjugated to magnetic beads, allowing the user to perform pull-down experiments without the traditional conjugation step. Unlike antibody-based protein precipitation methods, the aptamer-based method recognizes just the native form of target proteins, not the denatured form. The mild elution conditions used with the kits enable isolation of intact proteins and protein complexes. The aptamer-conjugated magnetic beads can be regenerated and reused several times for AP/Co-AP assays without any significant loss in binding capacity.

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