Related to Differentiating Mitochondrial Toxicity

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• Cell-Based Kinase Assays
• Cisbio Bioassays
• Three kinase assays are now available as part of a cell-based kinase screening platform. Phospho-STAT3 (signal transducer and activator of transcription 3) (Tyr705), Phospho-p38 MAPK (Thr180/Tyr182), and Phospho-IKKβ (Ser177/Ser181) involve targets
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• Retinoic Acid Assay Kits
• INDIGO Biosciences
• Retinoic acid receptor (RAR) gamma and retinoic-acid orphan receptor (ROR) gamma assay systems are now available in cell-based assay kits that enable researchers to obtain data in four steps: thaw, feed, dose, read. The RORγ and RARγ assay kits can
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• Bioluminescent Enzyme
• Promega
• The NanoLuc™ luciferase can provide new capabilities in reporter assays with the potential to aid with biologically complex applications that require greater sensitivity. It is small in size, allowing for viral delivery and protein fusion applicati
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• Microplate Reader
• BioTek Instruments
• Synergy™ NEO HTS multimode microplate reader is tailored for smaller cell-based screening assays. Synergy NEO includes multiple parallel detectors to decrease measuring time, and filter-based optics for live-cell assays. The Hybrid Technology™ foun
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• ssDNA Quantitation
• Promega
• The QuantiFluor™ ssDNA system is designed for highly sensitive quantitation of single-stranded DNA (ssDNA). The QuantiFluor ssDNA dye enables quantitation of small amounts (as little as 200 pg per well) of ssDNA in solution. For low-concentration s
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• Resistant-to-Inhibitor Master Mixes
• Promega
• The GoTaq® Probe PCR Master Mix, GoTaq Probe 1-Step RT-qPCR system, and GoTaq Probe 2-Step RT-qPCR system are ready-to-use, 2x master mixes that can simplify the preparation of reactions for qPCR using hydrolysis probe detection. They are desi
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• DNA Isolation
• Tecan
• The Freedom EVO®-HSM workstation provides walkaway extraction of gDNA from large-volume blood samples using Promega’s ReliaPrep™ large-volume HT gDNA isolation system. The workstation is designed to streamline biobanking workflows, offering on
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• Transporter Knockout Cell Lines
• Sigma-Aldrich
• MDR1, BCRP, and MRP2 transporter knockout cell lines are now available that enable identification of substrate/transporter interactions without chemical inhibitors or knockdown RNAi technology. The knockout cell lines are available as assay-ready p
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• Incubator for Cell-Based Research
• Thermo Fisher Scientific
• The Cytomat® 10 is an automated incubator and storage module for high-capacity cell growth and assay incubation. It offers environmental control and can be integrated with existing automated assay hardware. A thermal disinfection method can ge
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• Bisulfite Conversion System
• Promega
• The MethylEdge™ bisulfite system offers a method for performing bisulfite conversion and DNA clean-up while maintaining DNA integrity to provide intact, converted DNA for a range of sensitive applications. MethylEdge requires small amounts of input
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• Mitochondrial Toxicity Assays
• INDIGO Biosciences
• A new series of assays called the MitoVir platform evaluate mitochondrial toxicity by examining inhibition of the ribonucleoside triphosphates that are substrates for the human mitochondrial RNA polymerase (POLRMT). The MitoVir platform is based on
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• Protein Proteolysis Mix
• Promega
• Trypsin/Lys-C mix is a combination of Trypsin Gold and rLys-C proteases. The mix increases peptide generation with in-solution protein digestion and can work with difficult-to-digest proteins. Enhanced proteolytic activity achieved through use of T
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• Cytotoxicity Assay
• Promega
• The Mitochondrial ToxGlo™ assay helps produce the predictive data necessary to assess mitochondrial function in the context of other cytotoxic events. Based on the differential measurement of biomarkers associated with changes in cell membrane inte
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• Reporter Kits
• Horizon Discovery
• Available through a partnership with Promega, X-MAN™ reporter kits can enable researchers to apply cell-based assays that directly interrogate critical biological activities at endogenous expression levels. The X-MAN reporter kits are designed for