Related to 7 Tips for Optimal DNA Transfection

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• Rat Transgene Integration
• Sigma-Aldrich
• The CompoZr® Targeted Integration Kit-rRosa26 integrates transgenes into the Rosa26 safe harbor locus in the rat genome. CompoZr targeted integration kits employ CompoZr zinc finger nucleases (ZFNs) for precise and heritable gene knock-in, ena
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• DNA and RNA Purification
• MP Bio
• RapidGene-12™ is an automated nucleic acid purification instrument for the extraction and purification of DNA and RNA in less than 45 minutes. Based on magnetic bead technology, the instrument is compact and designed for routine daily laboratory wo
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• DNA Ladder
• G-Biosciences
• DNAmark™ Logic Marker contains 16 DNA bands, ranging from 100 base pairs to 10 Kb. The DNA ladder is formulated so that each band contains an amount of DNA that correlates logically to its size, allowing the user to estimate both the size and the q
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• DNA Polymerase
• New England BioLabs
• The Q5™ high-fidelity DNA polymerase can provide high- fidelity amplification while also offering amplification across a range of amplicons, including those that are GC- and AT-rich. This polymerase is fused to the processivity-enhancing Sso7d DNA
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• ssDNA Quantitation
• Promega
• The QuantiFluor™ ssDNA system is designed for highly sensitive quantitation of single-stranded DNA (ssDNA). The QuantiFluor ssDNA dye enables quantitation of small amounts (as little as 200 pg per well) of ssDNA in solution. For low-concentration s
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• CHO Cell Cloning
• STEMCELL Technologies
• The ClonaCell™-CHO ACF supplement improves cloning efficiency of CHO cells during cell-line development. The challenges of low cloning efficiency can result in significant waste of time and resources during single-cell cloning. The ClonaCell-CHO AC
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• Cell-Line Customization Service
• Cellectis bioresearch
• Gene customization experts can modify cell lines at any gene, at any position. Services available include gene modification, knock-out, and targeted transgene integration. Thirteen years of genome customization expertise, TALEN™ technology, and IP
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• Dendritic Cell Generation
• HumanZyme
• The HumanKine G4 dendritic cell generation kit can yield DCs from peripheral blood mononuclear cells with no medium changes. This protocol is enabled by HumanKine GM-CSF and HumanKine IL-4, which are included in the kit and produced in a human cell
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• Sample Quality Control
• Agilent Technologies
• The Genomic DNA ScreenTape can work with the 2200 TapeStation instrument to determine the quantity, size, and quality of genomic DNA in less than two minutes per sample, using only one microliter of material. This automated sample QC tool can be us
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• Bisulfite Conversion System
• Promega
• The MethylEdge™ bisulfite system offers a method for performing bisulfite conversion and DNA clean-up while maintaining DNA integrity to provide intact, converted DNA for a range of sensitive applications. MethylEdge requires small amounts of input
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• Plant and Seed DNA Isolation Kits
• Mo Bio Laboratories
• Two new kits are available with Inhibitor Removal Technology to remove polysaccharides, polyphenolics, and lipids for pure DNA. The PowerPlant Pro-htp 96-Well Kit enables high-throughput isolation of pure DNA from plants and seeds in a 96-well form
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• Transfection Reagent
• Mirus Bio
• TransIT®-BrCa transfection reagent is a dedicated breast cancer cell-line specific reagent that can help researchers achieve high expression levels in breast cancer cell types including MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, and T47D. It c
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• iPS Cell Lines
• Cellectis bioresearch
• The iPS Engineering Hub™ service can provide users with customized iPS cell lines engineered with TALEN™ technology. Donated somatic cells are reprogrammed by means of co-expressing four factors (Oct-4, SOX2, c-Myc, and Klf-4) by nonintegrating tec