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March 1, Vol. 35, No. 5

  • Feature Articles

    • Untangling the Human Proteome
    • MaryAnn Labant
    • More complex than protein identification, protein quantification requires extra attention to detail Routinely used, mass spectrometers will continue to get faster, more sensitive, and more accurate, and will eventually evolve to a single acquisition method for both qualitative and quantitative ... more »
    • Digital PCR for Medicine
    • Neil McKenna, Ph.D.
    • The revolution in nucleic acid detection and quantification heralded by the invention of polymerase chain reaction (PCR) in the late 20th century greatly advanced the field of molecular diagnostics Classical PCR is used to qualitatively or quantitatively interrogate a target ... more »
    • Affinity Labels for Protein Purification
    • Angelo DePalma
    • Labile, poorly expressed, or poorly soluble proteins paose many difficulties in isolation and purification These difficulties can be overcome, however, by various means One approach is to modify recombinant proteins by means of fusion tagging, the addition of amino acids, functional ... more »