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December 1, Vol. 34, No. 21

  • Feature Articles

    • Genomic Singularity Is Near
    • Kate Marusina
    • The explosive growth of DNA-based diagnostics empowers us to take a closer look at our own health and to demand answers from healthcare providers to questions that have never been asked before... more »
    • GEN Roundup on Flow Cytometry
    • Flow cytometry has long been used in cell counting and cell sorting More recent applications include biomarker detection, protein engineering, cell signaling, pathway screening, and systems biology GEN recently interviewed a number of flow cytometry researchers and specialists to get ... more »
    • Special Report: Microarray Not Fade Away
    • Lisa Heiden
    • The microarray, the legacy technology for measuring gene expression, will not go gentle into that good night True, in some respects, the microarray will be eclipsed by next-generation sequencing (NGS), specifically, whole transcriptome shotgun sequencing But the microarray will remain ... more »
  • Columns

    • Refining Cellular Assays via Micropatterns
    • Gail Dutton
    • French contract research organization (CRO) Cytoo specializes in developing high-content, three-dimensional cellular assays for muscle and skin It does so by means of micropatterns, which are initiated photolithographically and are ultimately elaborated at the cellular level According to Cytoo, micropatterns ... more »
    • FDA’s Supply Chain Initiative
    • Title VII of the US Food and Drug Administration’s Safety and Innovation Act (FDASIA) of 2012 seeks to quell the invisible hand that until now has guided sourcing and supply of raw ingredients and drug substances The law, which is ... more »
    • Monitoring Cell Migration and Invasion
    • Leyna Zhao, Ph.D.
    • Cell migration and invasion are fundamental components of embryogenesis, vasculogenesis, immune responses, and pathophysiological events such as cancer cell metastasis Cell migration and invasion involve morphological changes resulting from actin cytoskeleton rearrangement and the emergence of protrusive membrane structures followed ... more »
  • Tutorials

    • Conversion of ELISA to SMC™ Technology
    • Biomarker concentrations are a key determinant measurement in many aspects of the drug discovery and development process Specific biomarker measurements are key components for discovery, translational research, and safety assessment to name a few For preclinical and clinical samples, the ... more »
    • Comprehensive DNA Quantitation
    • Fabiola Saffache
    • With the advent of new technologies that aim to more clearly discern subtle and complex information from small samples, the requirement for setting quality control processes has never been greater The need to determine sample concentration has particularly expanded in ... more »
    • Lysis and Purification of Recombinant Protein
    • Anja Dedeo
      Jesmi George
    • Traditionally, protein purification from E coli includes four phases: harvest, bacterial cell lysis, lysate clarification, and protein purification Bacterial lysis typically requires several time-consuming steps, including freeze/thaw cycles and sonication, which may negatively impact protein quality and contribute to sample ... more »