Isolation of candidate mammalian cell clones by limiting dilution, ring cloning, or simple manual collection of colonies is a time-consuming, resource-intensive, and costly procedure that is prone to cross contamination of cells and user error. One major disadvantage commonly encountered is the difficulty in establishing monoclonality—an essential step in the production of therapeutic proteins and antibodies.
To overcome many of the drawbacks typically associated with isolating choice mammalian cell clones, Genetix has developed the ClonePix™ FL system for screening and selection of secretory cell lines. The utility of ClonePix FL lies in its ability to visualize and quantify proteins secreted from thousands of clones in situ, and to select and accurately isolate only the highest value secretors, shortening time scales and simplifying downstream culture.
The ClonePix FL solution has been validated for specific detection of secreted antibodies and monomeric proteins, cell surface proteins, and intrinsic GFP fusion proteins. It is compatible with a range of cell types including hybridoma, myeloma, HEK293, and both suspension-adapted and adherent CHO cells.