Studying Drug-Membrane Interactions
The ProteOn Liposome Capturing Kit provides a novel hydrophilic surface chemistry that is used to capture intact lipoparticles, vesicles, and lipid assemblies with low non-specific binding, easy surface regeneration, and the ability to capture multiple layers of lipid assemblies for increased sensitivity.
The ProteOn LCP sensor chip surface is saturated with single-stranded biotinylated DNA molecules from the Liposome Capturing Kit, which enables liposomes tagged with cholesterol-labeled double-stranded DNA molecules to be captured to the surface through DNA hybridization.
This kit has been used to measure the binding of small molecule drugs to a variety of lipid membranes captured to the ProteOn LCP sensor chip surface (Figure 1). This type of analysis is important in drug discovery and development where drugs with ideal efficacy should be both soluble in an aqueous environment and able to pass through lipophilic barriers such as cell membranes. This balance between hydrophilicity and lipophilicity is an essential aspect to consider in drug discovery.
Many other in vitro methods have been developed, but only a few accurately predict drug transport across biological membranes. Researchers have been analyzing drug partitioning using liposomes, which mimic the lipid bilayer geometry and ionic characteristics of a biological membrane.