In order to provide biopharmaceutical developers with more information at an earlier stage low sample volume or scale-down analytical technologies are required. The Optim 1000 developed by Avacta Analytical has been designed to provide information about conformational stability and aggregation propensity with high throughput and using small amounts of protein, with the added benefit of fully automated operation, data analysis, and reporting.
The development of a specialized sample holder or microcuvette array (MCA) allows the system to analyze up to 48 samples in one run using as little as 1 µL of protein solution in each sample. Depending on the sample and application this can mean using concentrations to below 0.1 mg/mL, corresponding to 100 ng of protein per sample.
The MCA permits high-quality fluorescence and light-scattering data to be recorded from these small volumes, and thermoelectric heating and cooling allows the samples to be subjected to a thermal ramp or held at a fixed temperature during analysis.
In the basic instrument the samples are analyzed by two techniques simultaneously: intrinsic protein fluorescence spectroscopy is used to monitor protein tertiary structure and static light scattering is utilized to monitor protein aggregation. In addition, extrinsic fluorescent dyes can be added to the sample to give complementary information about protein conformation and aggregation. These analytical techniques are particularly well suited to rapid, sensitive analysis of small volumes of protein in the widest range of possible buffer or solvent conditions.
In a typical experiment to elucidate stability and aggregation propensity, the protein samples are heated while their intrinsic fluorescence and light-scattering signals are simultaneously monitored. Figure 1 shows typical output of such an experiment.
Changes in the fluorescence emission (Figure 1A) show the loss of higher order structure and that the protein is thermally denatured. By monitoring this signal, Optim can determine a temperature midpoint, Tm, of the unfolding transition (Figure 1B). The scattered light intensity increases strongly when aggregates are formed and this can be characterized using a second parameter, the aggregation onset temperature, Tagg (Figure 1C).
These parameters can be used to screen different candidate proteins or explore the effect of different buffer conditions and additives on protein structure and aggregation. The data generated is comparable to that produced by alternative technologies such as differential scanning calorimetry, circular dichroism, and dynamic light scattering, but Optim is typically much faster and uses orders of magnitude less protein.