The versatility of the mosquito X1 is of value to small academic research groups that need to get maximal use and value from each instrument in their laboratory. Although mosquito HTS is more commonly employed to perform serial dilutions of active compound leads for dose-response curves, Larsen’s group successfully uses the single tipped X1 to perform serial dilutions of each hit compound selected. This group has found that with easy to use, dial-in volumes and transfer protocols, mosquito X1 can easily perform sequential pipetting with minimal re-programming.
Figure 1 demonstrates the results of an eight-point dilution series of 10 hit compounds on a transiently expressed protein target. In this instance, dose-response curves were successfully achieved with mosquito X1 using lower volumes compared to those used in earlier studies. In addition, single disposable tip usage can be maximized, transferring multiple samples via sequential pipetting of the same sample, saving tip usage and costs.
This research group also used their mosquito X1 to set up control wells containing identical concentrations of unstable control reagents or low volumes of DMSO identical to those in the compound sample wells. Of greater importance, they also found that mosquito X1 (Figure 2) was especially useful for dry spotting compounds that are labile in aqueous solution.
Dry spotted compound plates were successfully stored at -20°C, ensuring sample integrity and providing assay-ready plates when required. The ability to pre-prepare plates at this secondary screening stage was highlighted to be of significant benefit to their workflow, while establishing optimal conditions for the set up of transient transfections.