Horizon Discovery’s virally mediated Genesis™ genome-editing technology enables targeted engineering of endogenous genes in human cells lines to create genetically defined knock-out or knock-in cellular models. Homology cassettes carried in recombinant adeno-associated virus (AAV) vectors are used to perform targeted homologous recombination, ensuring no off-target integrations or sequence errors.
Horizon has applied this technology to create X-Man™ patient-relevant human cell lines for personalized medicine applications, designed to model the disease-causing mutations present in a patient’s tumor. These patient-specific models can be used to study drug activity, therapeutic response, and drug resistance, and to design individualized treatment regimens and select patients for clinical studies.
The company recently joined the European Union Colon Cancer and Therapeutics (COLTHERES) consortium and is generating X-Man colon cancer cell lines that incorporate predictive biomarkers of drug response and resistance, as well as diagnostic and prognostic biomarkers.
Johannes Ottl, unit head of label-free technologies at Novartis Pharma, presented a poster entitled, “SPR in early phases of low molecular weight lead discovery,” in which he described the use of surface plasma resonance (SPR) in screening campaigns for hit identification and for validation and characterization of binding, including affinity and kinetic measurements, of low molecular weight compounds to a target.
Ottl’s group is using chip-based SPR technology from Bio-Rad that allows for screening multiple protein samples in parallel, which he notes, offers advantages for running replicates and evaluating compound selectivity and modified/mutated proteins side-by-side in the same experiment.
Bio-Rad’s ProteOn™ XPR36 Protein Interaction Array System allows users to immobilize a panel of 6 ligands on the surface of a 6x6 chip and to measure 36 interactions at one time. The chip surface is composed of a modified alginate polymer layer bound to the gold surface of the sensor prism. Bio-Rad recently introduced the ProteOn HTG sensor chip, containing tris-NTA complexes immobilized to the gold surface to capture His-tagged proteins.
The SoPRano™ SPR-based label-free high-throughput screening platform from Pharma Diagnostics designed for performing protein binding assays, enables localized SPR detection on gold nanoparticles and is compatible with absorbance at 530 nm on standard plate readers. The initial SoPRano kit utilized passive charge absorption coupling chemistry. The company recently launched kits with biotin-direct coupling chemistry and carboxyl covalent coupling for stable conjugation of amine-containing molecules.
HighRes Biosolutions’ new TCell™ table-based automation system offers a variety of enclosure options, including BSL2, HEPA filtration, laminar flow, and temperature and humidity control. It contains three-axis device platforms for ease of access to instruments, the Cromium robot powered by a harmonic drive gearing system, under-table storage, and an optional MicroDock for quick switching of additional devices such as readers, incubators, or pipettors.
The Picodrop 200 spectrophotometer from Micronic North America performs protein or DNA quantification in 2 µL samples in a patented disposable pipette tip with a three-second read time. The device uses a UV xenon light source and can read across a wavelength range of 220–950 nm.
Later this year, a collaborative venture between Tecan and HP will bring to the drug screening market dispensing products based on HP’s inkjet technology.