As an example, DBCs measured at 10% breakthrough are equal to 65, 55, 35, and 30 mg/mL at 250, 500, 1,000 and 1,500 cm/hr, respectively (Figure 1). Columns of 5 mm I.D. and 100 mm bed height were packed with AbSolute High Cap.
DBCs at 10% breakthrough also were determined for AbSolute High Cap and several industry standards with human IgG in PBS at 0.5 mg/mL. All these media were packed in columns of 5 mm I.D. and 50 mm bed height (bed volume of 0.98 mL).
AbSolute High Cap illustrated a higher DBC at 10% breakthrough than many market standards at all linear velocities (Figure 2). As an example, using AbSolute High Cap compared to the third-generation agarose-based media at 90, 150, and 375 cm/hr, the overall performance is improved by 100%, 125%, and 230% respectively.