Researchers in the areas of regenerative medicine, basic biomedical research, and drug discovery all share a common need for reproducible procedures to culture human pluripotent stem cells (hPSCs) and/or differentiated cells. Some of the technical steps involved in this include derivation of new human embryonic and induced pluripotent stem (iPS) cell lines, expansion to large quantities, differentiation to appropriate cell types, and finally maintaining the differentiated cells in their matured state. All these steps must comply with clinical requirements.
Many researchers still believe there are major challenges involved in almost all above mentioned steps. Derivation of stem cells in a clinically relevant manner has been difficult if not impossible. Expansion of stem cells has resulted in karyotypic changes or unwanted differentiation. Directed differentiation has been challenging with more than 50,000 researchers searching for answers. When mature cells are placed in vitro for culture, they often de-differentiate within hours to days into other cell types, such as fibroblasts.
In this article I will discuss the use of biologically relevant human recombinant laminins from BioLamina that successfully recreate specific cell niches in the cell culture dish. This enables robust cell cultivation in highly physiological microenvironments that practically solves all current technical challenges with stem cell culture.