Next-Generation Sequencing (NGS) Innovations
Swift Biosciences is developing a suite of technology platforms to improve NGS sample preparation, an area that has not kept pace with recent improvements in instrumentation and bioinformatics. These platforms are based on proprietary protocols and reagents invented by Swift. Among the applications under development:
- DNA library creation
- Targeted selection
All of these applications share the features of being fast, easy and compatible with existing NGS instrumentation.
In February 2013, Swift launched the first product in a new line of kits for NGS sample preparation, Accel-NGS™ DNA Library Kit. NGS users can now produce PCR-free libraries with as little as 5 ng of input DNA. The highly efficient Swift adaptation technology eliminates the need for PCR, thereby minimizing base composition bias and fidelity issues while reducing the input requirement. The unique, two-step adaptation process also reduces adapter dimer formation to maximize sequencing output. Unlike other kits, the Accel-NGS DNA Library Kit does not require intact double-stranded DNA, making it ideal for FFPE and damaged samples.
The Accel-NGS DNA Library Kit protocol is fast, requiring only 75 minutes start-to-finish, and consists of 5 easy steps, two of which are bead-based separations that eliminate the need for time-consuming, electrophoretic gel-based size selection. In addition, the streamlined workflow can be readily automated.
Accel-NGS products for RUO applications are available directly from Swift. Additional NGS sample preparation products will be launched in the near future.