The Pfenex Expression Technology™ platform is a very powerful protein expression technology based on the microorganism Pseudomonas fluorescens. The Pfenex platform technology can deliver a robust production strain with unprecedented speed and a success rate that transcends legacy production hosts.
Traditional recombinant protein expression hosts such as E. coli, insect cells, or yeast tend to have high failure rates regarding their ability to produce high titers of soluble, full-length active target protein. In addition, the time required to identify the optimal expression strain in these systems, due to a linear and iterative approach to strain engineering, is protracted, resulting in significant opportunity cost for the drug developer.
Pfenex Expression Technology dramatically reduces strain development time and hence product development time via robotically enabled high-throughput, parallel-expression strain screening. The resultant selected production strain delivers a lower overall cost of goods associated with rapid fermentation cycle times, higher cell densities, and low cost media. The combination of speed, success rate, and reduced cost of goods has established Pfenex Expression Technology as the premier technology for recombinant protein production.
The Pfenex Expression Technology platform offers a robust solution to the real and opportunity costs incurred through the maintenance of several recombinant expression platforms. Even with smaller scale programs, the utmost importance is placed on speed, while not sacrificing quality and quantity of protein delivered.
Performance data derived from traditional expression hosts suggest development program delays in the range of 5 to 12 months on programs that have utilized such a serial approach.
The real costs of such delays, including materials, FTEs, and facility costs are in the millions of dollars, and the delays of a product reaching the commercialization stage could certainly add millions of dollars more to that total.
As a result, utilizing the proven parallel-processing methods of the Pfenex Expression Technology platform in discovery and throughout development provides tangible benefits in expediting programs by efficient protein production, allowing programs to avoid attrition due to inability to produce product, and delivering a reduced long-term cost of goods that provides a sustainable competitive advantage against other competing products.