January 1, 2007 (Vol. 27, No. 1)

URL:
http://tools.neb.com/nebcutter2/index.php

Rating:
Strong Points: Useful free resource
Weak Points: None

Summary:
Here’s a first. I got a letter from a Nobel Laureate who reads the column suggesting that I review this site. It turned out to be a good one, so I decided to cover it for “Best of the Web.” NEBcutter has been out there for quite a while, and though I’ve mentioned it in passing before, I’ve never written it up. What NEBcutter does is identify open reading frames and restriction enzyme sites (with a few interesting wrinkles) within DNAs of up to 1 million bp in length. Hosted by New England Biolabs, the site offers numerous options, including open reading frame selections, plus quite a few different enzyme sets for the picking. It’s free, it’s fast, and it will provide anyone needing simple DNA sequence analysis with tools they can use. Definitely worth a bookmark.

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