The acquisition and processing of digital images is an essential aspect of biological research. While it is easy to learn how to acquire images with one’s lab microscope, that it not the same as gaining an understanding of the underlying principles of image acquisition and optimization. Fortunately, the Hamamatsu Learning Center provides a great number of free resources designed to teach researchers basic concepts in digital imaging technology. The site contains a number of interactive Flash/Java tutorials that cover topics such as dynamic range, image processing, resolution, and analog-to-digital conversion. Additional topics such as binning and quantum efficiency are discussed in some of the many review articles found on the site. So if you find yourself wondering about bit depth and image histograms, head on over to the Hamamatsu Learning Center to have all of your questions answered.