DNA sequencing has become an immensely useful and highly utilized technology in biological research. Yet, just like any other technique, DNA sequencing comes with its own set of technical hiccups that demand troubleshooting by the researcher. Luckily, researchers need not fret and stew in isolation when their sequencing reactions don’t go as planned. Rather, they can visit the DNA Sequencing Troubleshooting Web Resource made available by the Association of Biomolecular Resource Facilities (ABRF). Visitors to the site can browse the entire collection of troubleshooting issues by leaving the query fields blank; alternatively, users can search by keyword or problem category. (Problem categories include instrument problem, sample problem, software problem or “other”.) So before you spend too much time pulling your hair out, check out this public forum to possibly get some useful tips from fellow scientists.
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